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对白细胞从流程的分析招聘基质细胞的影响
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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对白细胞从流程的分析招聘基质细胞的影响

DOI:
10.3791/52480-v

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11:30 min

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January 07, 2015

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, , ,
1School of Clinical and Experimental Medicine,University of Birmingham, 2College of Medical and Dental Sciences,University of Birmingham, 3School of Immunity and Infection,University of Birmingham

Chapters

  • 00:05Title
  • 01:28Assembling the Flow System
  • 04:44Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
  • 06:59Perfusion of Leukocytes Over Endothelial Cells
  • 07:55Recording Neutrophil Capture and Behavior
  • 09:47Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

发炎的内皮从流动招募白细胞的能力是由间充质基质细胞调节。我们描述两个体外结合原代人细胞，它可以用于从流动评估中性粒细胞募集和检查的作用是间充质基质细胞在调节这个过程中发挥的模型。

Tags

Keywords: Endothelial CellsLeukocyte RecruitmentNeutrophil AdhesionMesenchymal Stem CellsIn Vitro Vascular ModelFlow-based Adhesion AssayTNF-alphaInflammatory Regulation

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