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At analysere virkningen af stromacellers om ansættelse af Leukocytter fra Flow
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Chapters
- 00:05Title
- 01:28Assembling the Flow System
- 04:44Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
- 06:59Perfusion of Leukocytes Over Endothelial Cells
- 07:55Recording Neutrophil Capture and Behavior
- 09:47Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
- 11:06Conclusion
Evne betændt endotel at rekruttere leukocytter fra flow reguleres af mesenkymale stromale celler. Vi beskriver to in vitro modeller med primære humane celler, som kan anvendes til at vurdere neutrofil rekruttering fra flow og undersøge den rolle, som mesenchymale stromale celler spiller i reguleringen af denne proces.
