A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:17Injection of Thymidine Analogs and Tissue Preparation
- 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
- 06:27Imaging and Data Analysis
- 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
- 08:42Conclusion
This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.
