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Chapters
- 00:05Title
- 01:47Ovariole Dissection
- 03:25Ovariole Immunofluorescent Staining
- 05:36Glycerin Jelly Slide Preparation
- 06:57Egg Chamber Mounting
- 09:22Egg Chamber Imaging Preparation
- 10:35Results: Enhanced Imaging with the Upright Technique
- 11:56Conclusion
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
