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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

DOI:
10.3791/52651-v

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09:26 min

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February 20, 2015

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, , , , , ,
1Departments of Pediatrics and Pharmacology,University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital,Third Military Medical University

Chapters

  • 00:05Title
  • 01:28Stimulation and Collection of Microparticles
  • 03:26Characterization of Microparticles via FACS Analysis
  • 05:10Preparation of Bronchial Tissue Explants
  • 06:44Lymphocytic Microparticle Treatment
  • 07:51Results: Lymphocytic Microparticle Detection and Analysis
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

Tags

Keywords: Lymphocyte MicroparticlesCell-cell CommunicationApoptosisAirway Epithelial CellsCell Death Detection

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