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Oxigênio-Glucose Privação e reoxigenação como um<em> In Vitro</em> Isquemia e reperfusão Lesão modelo para estudar Disfunção Barreira Hematoencefálica
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Medicine
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JoVE Journal Medicine
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
DOI:

08:56 min

May 07, 2015

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Chapters

  • 00:05Title
  • 01:16Seeding Endothelial Cells (RBMECs)
  • 03:31Oxygen and Glucose Deprivation-Reoxygenation In Vitro Model
  • 04:40Immunofluorescence Localization and f-actin Labeling with Rhodamine Phalloidin
  • 07:29An Oxygen-Glucose Deprivation and Reoxygenation (OGD-R) Experiment
  • 08:22Conclusion

Summary

Automatic Translation

Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.

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