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عزل الخلايا الجذعية العصبية / السلف من المنطقة المحيطة بالبطين في الجرذان الكبار والحبل الشوكي البشري
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Chapters
- 00:05Title
- 01:29Harvesting and Dissection of Adult Rat Periventricular Spinal Cord Tissue
- 03:43Isolation and Culturing of Adult Rat Neural Stem/Progenitor Cells from the Spinal Cord
- 06:51Results: Isolated Neurospheres Express Ki67 and Nestin by Passage 3
- 07:50Conclusion
The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.
