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细胞周期进程的时间跟踪使用流式细胞仪,无需同步
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Biology
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JoVE Journal
Biology
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
Please note that all translations are automatically generated.
Click here for the English version.
细胞周期进程的时间跟踪使用流式细胞仪,无需同步
DOI:
10.3791/52840-v
•
08:52 min
•
August 16, 2015
•
Robert Welschinger
,
Linda J. Bendall
1
Centre for Cancer Research
,
Westmead Millennium Institute for Medical Research and University of Sydney
Chapters
00:05
Title
01:30
Pulse Labeling of Cells with BrdU
03:41
Cell Fixation and Permeabilization
04:37
Antibody Staining and DNA Staining
06:12
Results: Flow Cell Analysis
08:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个协议描述了使用溴脱氧尿苷(BrdU)标记的摄取,以允许细胞是在S期在时间上的特定点的时间的跟踪。此外DNA染料和抗体标记的方便了S期细胞的命运在稍后时间详细分析。
Tags
Cell Cycle
Flow Cytometry
BrdU
Cell Synchronization
Temporal Tracking
DNA Synthesis
Cell Cycle Phases
Cell Cycle Progression
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