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형광 생체막 포스 프로브 : 하나의 세포에서 수용체 - 리간드 속도론의 동시 정량 및 바인딩에 의한 세포 내 신호
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
DOI:

14:09 min

August 04, 2015

, , , , , ,

Chapters

  • 00:05Title
  • 02:28Human RBCs Isolation, Biotinylation and Osmolarity Adjustment
  • 04:07Preparation for Micropipettes and a Cell Chamber
  • 06:24BFP Experiment
  • 10:25Fluorescence BFP (FBFP) Experiment
  • 11:51Results: Representative T-cell Receptor and Peptide MHC Binding Kinetics Data & Calcium Fluorescence Data from the FBFP Experiment
  • 13:14Conclusion

Summary

Automatic Translation

We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.

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