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Chapters
- 00:00Title
- 00:23Introduction
- 01:36Vesicle Preparation
- 04:22Bilayer Preparation
- 08:12Principles of Total Internal Reflection Microscopy
- 10:44Architectural of the Microscopy System
- 12:41Power Measurement
- 15:38Measurement Bilayer Fluidity
- 16:39Measurement Protein Density on the Bilayer
- 19:25Conclusion
Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.
