A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:59Passage of SH-SY5Y Maintenance Cultures
- 02:33Differentiation Procedures
- 06:24Results: Images of Cells in the Intermediate Steps of the Differentiation Protocol
- 07:28Conclusion
It is critical in neurobiology and neurovirology to have a reliable, replicable in vitro system that serves as a translational model for what occurs in vivo in human neurons. This protocol describes how to culture and differentiate SH-SY5Y human neuroblastoma cells into viable neurons for use in in vitro applications.
