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Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

DOI:
10.3791/59823-v

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09:00 min

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June 16, 2019

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, , ,
1Center for iPS cell research and application (CiRA)

Chapters

  • 00:04Title
  • 01:11Pluripotent Stem Cell Colony Formation
  • 02:45Hemogenic Induction and Isolation of Hemogenic Endothelium
  • 04:08Induction of Endothelial-to-hematopoietic Transition (EHT)
  • 05:00Flow Cytometry Analysis of Hematopoietic Cells
  • 06:27Colony Forming Unit Assay of Hematopoietic Cells
  • 07:30Results: Hemogenic Induction
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to precisely form hemogenic endothelium from human pluripotent stem cells in a feeder- and xeno-free condition. This method will have broad applications in disease modeling and screening for therapeutic compounds.

Tags

Hemogenic EndotheliumPluripotent Stem CellsFeeder-freeXeno-freeBlood CellsHematologyImmunologyGenetic InterventionGene EditingLM511-E8PSC ColoniesDissociation BufferDifferentiationHypoxiaCulture Conditions

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