A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:45Isolation of Skeletal Muscle
- 03:47Isolation of Mitochondria from Skeletal Muscle
- 07:50Mitochondria Membrane Integrity Assay
- 08:42Results: Membrane Integrity Measurements of the Isolated Preparation
- 09:31Conclusion
Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.
