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1Department of Cancer Biology,Dana-Farber Cancer Institute, 2Department of Pediatrics,Children’s Hospital, 3Department of Biostatistics & Computational Biology,Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research,Hebrew University of Jerusalem, 5Department of Neurosurgery,Children’s Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology,Dana-Farber Cancer Institute
Chapters
- 00:05Title
- 00:49Dissection
- 02:02Embedding the Brains in Agarose
- 03:28Slicing with the Vibratome
- 05:31Plating the Slices onto the Inserts
- 06:53Plating the Tumor Cells and Staining
- 08:57Results: Mouse Medulloblastoma Cells Treated with LDE225 (Sonidegib)
- 09:37Conclusion
Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.
Tags
Keywords: Brain TumorOrganotypic SliceCo-cultureTumor MicroenvironmentTargeted Drug TherapiesCancerMorbidityMortalityNeurosphere CulturesSerum-free ConditionsOrthotopic XenograftsPediatric Brain TumorsPilocytic AstrocytomasMedulloblastomasFluorescently Labeled Brain Tumor CellsDrug TreatmentsMicroenvironmentQuantitative Assay
