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Neuroscience

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Laser-guided Neuronal Tracing In Brain Explants
 

Laser-guided Neuronal Tracing In Brain Explants

Article DOI: 10.3791/53333-v 06:40 min November 25th, 2015
November 25th, 2015

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Summary

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

Tags

Keywords: Laser-guided Neuronal Tracing Brain Explants In Vitro Tracer Injection Electroporation Fluorescent Genetic Markers Band-pass Filter Goggles Neurocircuit Functionality
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