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Chapters
- 00:05Title
- 00:58Chemical Coupling of Proteins to Polymer Beads
- 04:49Use of Bead-coupled Adhesins in Competition Assays
- 07:04Measurement of Cytotoxicity
- 08:42Measurement of Bacterial Adhesion
- 10:17Results: Biomimetic Materials to Characterize Bacteria-host Interactions
- 11:51Conclusion
Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.
