/

Developmental Biology

/

蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Please note that all translations are automatically generated. Click here for the English version.

蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

DOI:

10.3791/53654-v

•

11:25 min

•

February 22, 2016

•

Nestor Saiz, Minjung Kang, Nadine Schrode, Xinghua Lou, Anna-Katerina Hadjantonakis

¹Developmental Biology Program,Sloan Kettering Institute

Chapters

00:05Title
00:41Embryo Collection
03:25Confocal Imaging
04:47Image Analysis and Data Pre-processing
08:44Results: Lineage Specification Analysis in Mouse Preimplantation Embryos
10:30Conclusion

Summary

Automatic Translation

该协议提出对蛋白质表达的原位分析进行定量，单细胞研究在小鼠植入前胚胎沿袭规范的方法。必要的囊胚的汇编程序，整个安装蛋白的免疫检测，在一个共焦显微镜，和核分割和图像分析的样品的成像进行描述。

Tags

Quantitative Analysis Protein Expression Lineage Specification Mouse Preimplantation Embryos Confocal Microscopy Single-cell Resolution Semi-automated Nuclear Density Cell Population Heterogeneity Zona Pellucida Removal Immunofluorescence Confocal Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎

在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响

斑马鱼视网膜蛋白定位的相关超分辨和电镜观察

从小鼠胚胎植入前胚胎干细胞系的推导

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

通过检测全小鼠胚胎β乳糖酶活性追踪基因表达

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程

小鼠皮肤角质细胞的分离和染色,用于细胞周期特定分析细胞蛋白表达的量度细胞测定

使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质

Read Article