JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
Please note that all translations are automatically generated.
Click here for the English version.
蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
DOI:
10.3791/53654-v
•
11:25 min
•
February 22, 2016
•
Nestor Saiz
,
Minjung Kang
,
Nadine Schrode
,
Xinghua Lou
,
Anna-Katerina Hadjantonakis
1
Developmental Biology Program
,
Sloan Kettering Institute
Chapters
00:05
Title
00:41
Embryo Collection
03:25
Confocal Imaging
04:47
Image Analysis and Data Pre-processing
08:44
Results: Lineage Specification Analysis in Mouse Preimplantation Embryos
10:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议提出
对
蛋白质表达的
原位分析
进行定量,单细胞研究在小鼠植入前胚胎沿袭规范的方法。必要的囊胚的汇编程序,整个安装蛋白的免疫检测,在一个共焦显微镜,和核分割和图像分析的样品的成像进行描述。
Tags
Quantitative Analysis
Protein Expression
Lineage Specification
Mouse Preimplantation Embryos
Confocal Microscopy
Single-cell Resolution
Semi-automated
Nuclear Density
Cell Population Heterogeneity
Zona Pellucida Removal
Immunofluorescence
Confocal Imaging
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/53654?article_type=v
损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎
check_url/53654?article_type=v
在
嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的
体内
成像来研究细胞非自治的影响
check_url/53654?article_type=v
斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
check_url/53654?article_type=v
从小鼠胚胎植入前胚胎干细胞系的推导
check_url/53654?article_type=v
小鼠胚胎心肌内祖群的定量全贴免疫荧光分析
check_url/53654?article_type=v
通过检测全小鼠胚胎β乳糖酶活性追踪基因表达
check_url/53654?article_type=v
用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板
check_url/53654?article_type=v
成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程
check_url/53654?article_type=v
小鼠皮肤角质细胞的分离和染色,用于细胞周期特定分析细胞蛋白表达的量度细胞测定
check_url/53654?article_type=v
使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质
Read Article