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Biology
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JoVE Journal Biology
High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
DOI:

09:27 min

May 10, 2016

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Chapters

  • 00:05Title
  • 00:33Plant Growth and Fixation
  • 02:17Dehydration and Infiltration
  • 03:25Block Preparation
  • 05:10Sectioning and Slide Preparation for Polarized Microscope
  • 07:11Results: Cell-type Specific Responses to Brassinosteroids in the Arabidopsis Root System
  • 08:21Conclusion

Summary

Automatic Translation

Crystalline cellulose is an important constituent of the plant cell wall. However, its quantification at a cellular resolution is technically challenging. Here, we report the use of polarized light technology and root cross sections to obtain information of cell wall composition at a spatiotemporal resolution.

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