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정성 및 정량 검출을위한 시험 법 및 단백질 항생제 특성
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
DOI:

10:50 min

April 10, 2016

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Chapters

  • 00:05Title
  • 00:42Qualitative Microslide Diffusion Assay
  • 03:37Substrate Labeling – Remazol Brilliant Blue R Dye Labeling
  • 05:38Quantitative Dye-release Assay
  • 08:20Results: Detection and Characterization of Novel Protein Antimicrobials
  • 09:56Conclusion

Summary

Automatic Translation

Here, we present protocols to rapidly screen and characterize enzymes for antimicrobial activity. The microslide diffusion assay and the dye-release assay utilize target bacterial substrates for qualitative and quantitative enzymatic activity evaluation.

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