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Chapters
- 00:05Title
- 01:06DNA Functional Quantification and Quality Control
- 02:55Library Preparation
- 05:22Library Purification and Size Selection
- 06:50Library Quantification
- 08:21Library Normalization and Sample Pooling
- 09:23Sequencing
- 11:09Results: High-depth, Multiplexed Sequencing and Sensitive Analysis of Challenging Cancer Specimens by Targeted NGS
- 13:01Conclusion
An integrated system for targeted next-generation sequencing of oncology specimens is described. This cross-platform system is optimized for low-quality and low-quantity tumor biopsies, accommodates low DNA inputs, includes well-characterized multi-variant controls, and features a novel variant caller that is informed by quantitative pre-analytical quality control measures.
