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心脏分化的表观遗传调控胚胎干细胞和组织
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
DOI:

13:03 min

June 03, 2016

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Chapters

  • 00:05Title
  • 01:10DNA-protein Cross-linking
  • 02:05Cell Lysis and Chromatin Fragmentation
  • 04:31Chromatin Immunoprecipitation
  • 05:49DNA Elution, Cross-link Reversal and Proteinase K Digestion
  • 07:33DNA Isolation Using DNA-binding Beads
  • 10:05Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
  • 11:47Conclusion

Summary

Automatic Translation

基因转录的微调调控underlies胚胎细胞命运决定。在此,我们描述了用于研究干细胞和小鼠胚胎的心脏发育的两个心脏分化的表观遗传调控染色质免疫沉淀测定法。

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