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Developmental Biology
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心脏分化的表观遗传调控胚胎干细胞和组织
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Developmental Biology
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JoVE Journal
Developmental Biology
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
Please note that all translations are automatically generated.
Click here for the English version.
心脏分化的表观遗传调控胚胎干细胞和组织
DOI:
10.3791/53874-v
•
13:03 min
•
June 03, 2016
•
Imen Jebeniani
,
Julia Leschik
,
Michel Puceat
1
INSERM UMR-910, Faculté de Médecine
,
Université Aix-Marseille
,
2
Institute of Physiological Chemistry
,
University Medical Center, Johannes Gutenberg University
Chapters
00:05
Title
01:10
DNA-protein Cross-linking
02:05
Cell Lysis and Chromatin Fragmentation
04:31
Chromatin Immunoprecipitation
05:49
DNA Elution, Cross-link Reversal and Proteinase K Digestion
07:33
DNA Isolation Using DNA-binding Beads
10:05
Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
11:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因转录的微调调控underlies胚胎细胞命运决定。在此,我们描述了用于研究干细胞和小鼠胚胎的心脏发育的两个心脏分化的表观遗传调控染色质免疫沉淀测定法。
Tags
Epigenetic Regulation
Cardiac Differentiation
Embryonic Stem Cells
Chromatin Immunoprecipitation
Gene Transcription
Embryonic Development
Cardiac Development
Epigenetic Marks
DNA
Embryoid Bodies
Cross-linking
Cell Fixation
Cell Lysis
Sonication
Protease Inhibitors
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