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Epigenetisk forordning af Cardiac Differentiering af embryonale stamceller og væv
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Chapters
- 00:05Title
- 01:10DNA-protein Cross-linking
- 02:05Cell Lysis and Chromatin Fragmentation
- 04:31Chromatin Immunoprecipitation
- 05:49DNA Elution, Cross-link Reversal and Proteinase K Digestion
- 07:33DNA Isolation Using DNA-binding Beads
- 10:05Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
- 11:47Conclusion
En finjustering regulering af gen transskription ligger til grund embryonal celle skæbne beslutning. Heri beskriver vi chromatin immunopræcipitationsanalyser anvendt til at undersøge epigenetisk regulering af både hjerte- differentiering af stamceller og kardial udvikling af musefostre.
