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的发展<em>体外</em>检测以确定间充质细胞将经历上皮 - 间质转化的收缩功能

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Developmental Biology

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JoVE Journal Developmental Biology

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Please note that all translations are automatically generated. Click here for the English version.

的发展<em>体外</em>检测以确定间充质细胞将经历上皮 - 间质转化的收缩功能

DOI:

10.3791/53974-v

•

06:02 min

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June 10, 2016

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Yu Mikami², Hirotaka Matsuzaki, Hideyuki Takeshima, Kosuke Makita, Yasuhiro Yamauchi, Takahide Nagase

¹Department of Clinical Laboratory,The University of Tokyo Hospital, ²Department of Respiratory Medicine,The University of Tokyo Hospital

Chapters

00:05Title
01:01Lung Epithelial Cell Preparation and Culture
01:55Epithelial-mesenchymal Transition (EMT) and Gel Contraction Assay
03:41Results: Representative EMT Confirmation
05:07Conclusion

Summary

Automatic Translation

Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

Tags

In Vitro Assay Mesenchymal Cells Epithelial-mesenchymal Transition (EMT)Contractility Inflammatory Cytokines Idiopathic Pulmonary Fibrosis Airway Remodeling A-549 Cells TGF-beta1 TNF-alpha Gel Medium 3D Culture

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