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Chapters
- 00:05Title
- 00:46Preparation of Cells in Extracellular Matrix
- 01:36Surrogate Preparation
- 03:51Surrogate Fixation and Processing
- 05:27Measuring Cell Density
- 06:39Results: Representative Images of a H&E-stained Section from Perfused and Solid Surrogates
- 07:37Conclusion
We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.
