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Chapters
- 00:05Title
- 00:39Preparation of a Single Cell Suspension from the Small Intestinal Intraepithelial Layer and Lamina Propria
- 05:22Preparation of a Single Cell Suspension from Peyer’s Patches
- 06:20Results: Intestinal Lymphocytes Have FSC and SSC Characteristics Similar to Splenocytes
- 07:10Conclusion
There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.
