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Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

DOI:
10.3791/54167-v

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11:44 min

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August 29, 2016

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, ,
1Department of Regenerative Medicine, Graduate School of Medicine,Yokohama City University, 2Department of Advanced Gastroenterological Surgical Science and Technology, Faculty of Medicine,University of Tsukuba, 3Regenerative Medicine Research Center,Jiangsu University Hospital

Chapters

  • 00:05Title
  • 00:59Generation of Acute Liver Injury Mouse Model
  • 02:52Preparation of Human Hepatic Stem Cells
  • 04:41Intrasplenic Transplantation of Human Hepatic Stem Cells
  • 06:25Detection of Transplanted Human Hepatic Stem Cell-derived Hepatocytes in the Mouse Liver
  • 08:13Results: Humanization of Mouse Livers using Human Hepatic Stem Cells
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.

Tags

Keywords: Humanized Mouse LiverHuman Hepatic Stem CellsCell TransplantationLiver ReconstitutionDiphtheria ToxinCell SortingCell CultureLiver InjuryCell DifferentiationDrug Metabolism

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