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Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
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Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

DOI:
10.3791/54257-v

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09:48 min

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July 27, 2016

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, , , , , , , , ,
1Division of Gastroenterology, Hepatology and Nutrition,Virginia Commonwealth University, 2McGuire Veterans Affairs Medical Center,Research BC-101

Chapters

  • 00:05Title
  • 00:48Exosome Removal from Plasma
  • 02:03Isolation of HDL by Density Gradient Ultracentrifugation
  • 05:47Desalting and Concentration of Lipoprotein Fractions
  • 06:55Results
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.

Tags

MiRNA IsolationHigh-throughputHigh Density LipoproteinHDLPlasmaExosome PrecipitationDensity Gradient UltracentrifugationVLDLLDLCardiologyHepatology

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