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Hurtig og forenklet metode til High Through-sætte Isolering af miRNA fra Highly Renset High Density lipoprotein
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JoVE Journal Biology
Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Hurtig og forenklet metode til High Through-sætte Isolering af miRNA fra Highly Renset High Density lipoprotein

DOI:
10.3791/54257-v

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09:48 min

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July 27, 2016

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, , , , , , , , ,
1Division of Gastroenterology, Hepatology and Nutrition,Virginia Commonwealth University, 2McGuire Veterans Affairs Medical Center,Research BC-101

Chapters

  • 00:05Title
  • 00:48Exosome Removal from Plasma
  • 02:03Isolation of HDL by Density Gradient Ultracentrifugation
  • 05:47Desalting and Concentration of Lipoprotein Fractions
  • 06:55Results
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

MikroRNA'er spiller en vigtig regulerende rolle og dukker op som nye terapeutiske mål for forskellige humane sygdomme. Det har vist sig, at miRNA transporteres i højdensitetslipoproteiner. Vi har udviklet en forenklet metode til hurtigt at isolere oprensede HDL egnet til miRNA analyse fra humant plasma.

Tags

Keywords: MiRNA IsolationHigh-throughputHigh Density LipoproteinHDLPlasmaExosome PrecipitationDensity Gradient UltracentrifugationVLDLLDLCardiologyHepatology

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