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核酸的分离过滤：一种简单快速的DNA提取方法

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Biology

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JoVE Journal Biology

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

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核酸的分离过滤：一种简单快速的DNA提取方法

DOI:

10.3791/54289-v

•

07:56 min

•

August 06, 2016

•

Sally M. McFall, Mário F. Neto, Jennifer L. Reed, Robin L. Wagner

¹Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering,Northwestern University, ²Pritzker School of Medicine,The University of Chicago

Chapters

00:05Title
01:08Preparation of FINA Sample Preparation Module
02:27Preparation of qPCR Tube
03:12Preparation of Contrived Specimen
04:00FINA Extraction
05:57Results: FINA Enables Efficient Detection of Proviral HIV DNA from Whole Blood
07:20Conclusion

Summary

Automatic Translation

在这里，我们描述了一个简单，快速的基于纸张的DNA提取的HIV前病毒DNA的全血通过定量PCR检测方法。这种协议可以在检测其他遗传标记或使用替代的扩增方法被扩展为使用。

Tags

Filtration Isolation Nucleic Acid Extraction DNA Extraction Rapid DNA Extraction Paper-based DNA Extraction FINA Method PCR Amplification HIV Proviral DNA Quantitative PCR Sample Preparation Module Blotting Pad Paraffin Film Glass Blood Separator Membrane Double-coated Polyester Diagnostic Tape PCR Tube

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