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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

JoVE Journal
Biology

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JoVE Journal Biology

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

DOI:

10.3791/54299-v

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08:30 min

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August 21, 2016

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Palak G. Patel², Shamini Selvarajah², Suzanne Boursalie², Nathan E. How², Joshua Ejdelman, Karl-Philippe Guerard, John M. Bartlett, Jacques Lapointe, Paul C. Park, John B. A. Okello², David M. Berman²

¹Department of Pathology & Molecular Medicine,Queen’s University, ²Division of Cancer Biology & Genetics, Queen’s Cancer Research Institute,Queen’s University, ³Department of Surgery, Division of Urology,McGill University, ⁴Transformative Pathology Program,Ontario Institute for Cancer Research (OICR)

Chapters

00:05Title
00:57Tissue Coring
02:40Deparaffinization of the FFPE Tissue Cores
03:45Homogenization of the Deparaffinized Cores
05:07Digestion with Proteinase K and Nucleotide Separation
06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
07:50Conclusion

Summary

Automatic Translation

This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.

Tags

RNA Extraction DNA Extraction Prostate Cancer Paraffin Film Punch Biopsy Xylene Centrifugation

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