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Herstellung von Formalin-fixierten und in Paraffin eingebetteten Gewebe Cores sowohl für RNA und DNA-Extraktion
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
DOI:

08:30 min

August 21, 2016

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Chapters

  • 00:05Title
  • 00:57Tissue Coring
  • 02:40Deparaffinization of the FFPE Tissue Cores
  • 03:45Homogenization of the Deparaffinized Cores
  • 05:07Digestion with Proteinase K and Nucleotide Separation
  • 06:12Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
  • 07:50Conclusion

Summary

Automatic Translation

Dieses modifizierte Extraktionsprotokoll verbessert RNA und DNA-Ausbeuten von gezieltere Regionen von Interesse in histopathologischen Gewebeblöcke.

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