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Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

DOI:
10.3791/54308-v

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06:28 min

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September 25, 2016

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, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Chapters

  • 00:05Title
  • 00:54Plating hPSC Aggregates in the Microwell Plate
  • 02:00Cardiac Induction Stage 1
  • 03:06Cardiac Induction Stage 2
  • 04:16Cardiac Induction Stage 3
  • 05:06Results: Characteristics of Differentiated hPSCs
  • 05:44Conclusion

Summary

Automatic Translation

Automatic Translation

Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.

Tags

Keywords: Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

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