Journal
/
Developmental Biology
/
Samlet Size optimering i Microwells for suspension-baserede Cardiac Differentiering af menneskelige pluripotente stamceller
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

Samlet Size optimering i Microwells for suspension-baserede Cardiac Differentiering af menneskelige pluripotente stamceller

DOI:
10.3791/54308-v

•

06:28 min

•

September 25, 2016

•

, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Chapters

  • 00:05Title
  • 00:54Plating hPSC Aggregates in the Microwell Plate
  • 02:00Cardiac Induction Stage 1
  • 03:06Cardiac Induction Stage 2
  • 04:16Cardiac Induction Stage 3
  • 05:06Results: Characteristics of Differentiated hPSCs
  • 05:44Conclusion

Summary

Automatic Translation

Automatic Translation

Konventionelle metoder til at initiere suspension aggregat baseret hjertedifferentiering af humane pluripotente stængler celler (hPSCs) er plaget med kultur heterogenitet med hensyn til den samlede størrelse og form. Her beskriver vi en robust metode til hjertedifferentiering anvender mikrobrønde at generere størrelse-kontrollerede hPSC aggregater dyrkes under hjerte--fremmende betingelser.

Tags

Keywords: Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54308?article_type=v

Stencil Micropatterning for Human pluripotente stamceller til Sondering Spatial Organisation af differentiering Fates
check_url/54308?article_type=v

Storstilet produktion af cardiomyocytter fra menneskelige pluripotente stamceller Ved hjælp af en meget reproducerbar lille molekyle-Based Differentiering Protocol
check_url/54308?article_type=v

Hurtig neuronal differentiering af induceret pluripotente stamceller til måling Network Aktivitet på Micro-elektrode Arrays
check_url/54308?article_type=v

Pluripotente Stem Cell Afledt hjerteceller til Myokardie Repair
check_url/54308?article_type=v

Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller
check_url/54308?article_type=v

In vitro differentiering af humane pluripotente stamceller til trofoblastceller
check_url/54308?article_type=v

Hurtig, instrueret differentiering af retinale Pigment epitel celler fra menneskelige embryonale eller induceret pluripotente stamceller
check_url/54308?article_type=v

Effektiv differentiering af humane pluripotente stamceller i leverceller
check_url/54308?article_type=v

I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller
check_url/54308?article_type=v

Hemogen endothelium differentiering fra humane pluripotente stamceller i en feeder-og Xeno-fri defineret tilstand

Read Article