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Novel-RNA-bindende eiwitten Isolatie door de snelle Methodologie
JoVE Journal
Genetics
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JoVE Journal Genetics
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
DOI:

11:19 min

September 30, 2016

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Chapters

  • 00:05Title
  • 00:36Growing Cells for RaPID
  • 01:52Preparing Cell Lysates for RaPID
  • 04:38RaPID Purification
  • 08:36Results: RaPID Enables the Isolation of a Specific mRNA with its Associated Proteins
  • 10:26Conclusion

Summary

Automatic Translation

RNA-protein interactions lie at the heart of many cellular processes. Here, we describe an in vivo method to isolate specific RNA and identify novel proteins that are associated with it. This could shed new light on how RNAs are regulated in the cell.

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