JoVE Journal
Biochemistry
Biochemistry
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Chapters
Summary
We describe here a method for identification of small molecule-binding proteins using photoaffinity labeling. The advantage of this technique is that binding and covalent labeling of the target proteins occurs within the live cellular environment, removing the risk of disrupting native protein structure and binding conditions upon cell lysis.
Tags
Keywords: Small Molecule Binding Proteins Photoaffinity Labeling Live-cell Molecular Mechanism Drug Target Protein Identification Native Cellular Environment
PLAYLIST
Pharmaceutical Chemistry Formulation of introductory drug. Generic and Ethical Drugs Pre-formulation: Introduction, influencing factors
Ethical drug development processes and generic drug action sites and interactions
Drugs with them: lipids, enzymes, receptors and nucleic acids solid formulations: tablets - considerations in formulation design, composition, production methods, coating, finished product tests, types of tablets
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Research • ChemistryMass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders -
Research • BiochemistryLaboratory Scale Production and Purification of a Therapeutic Antibody -
Research • BioengineeringTransport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study -
Research • BioengineeringGraphene Coatings for Biomedical Implants
Combinatorial Chemistry Semi-solid formulations: purpose of local use, creams, gels - considerations in formulation design, chemical composition, physicochemical properties, preparation methods - Drug Design: Determining Structure Ratio
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Research • BiologyA Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection -
Research • Immunology and InfectionNanomechanics of Drug-target Interactions and Antibacterial Resistance Detection -
Research • BiologyProtein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Active SAR Functional Groups (Pharmacopoeial Colloids: Difference between Colloidal Solutions, Colloid Types) Stability Emulsions: Types of Emulsions (Selection of HLB Composition and Properties, Milk Calculations The quantitative relationship between the drug's structure and its activity (QSAR) (types of surfactants, composition and properties, stability enhancement methods, substances) - Drug Design Strategies 8 Optimize interactions with the target site
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Research • BiochemistryIdentification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling -
Research • Immunology and InfectionA Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors -
Research • BioengineeringIn Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox -
Research • EnvironmentExtraction and Characterization of Surfactants from Atmospheric Aerosols
Liquid Formulations Considerations in the formulation design
Physical and chemical properties
Different types of solutions: aqueous solutions, non-aqueous solutions, solutions for local use
Isotonic solutions for injection
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Education • Core: BiologyTonicity in Animals -
Education • Lab BioDiffusion and Osmosis- Concept -
Research • BiologyMeasuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example -
Research • BiologyInjection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes -
Research • BiologySimple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection
Spray Medications: Composition, Advantages, Types
Packaging Prodrugs and Formulation
Opiate painkillers (nsaids) nonsteroidal anti-bacterial (antibacterial drugs) Need for controlled release drugs: Defined release versus controlled release, drug controlled release types, diffusion-based systems and transdermal systems Design and Synthesis of Drugs Microcapsulation Peptides for Drugs: Microcapsules
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Research • Immunology and InfectionFacilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish -
Research • BioengineeringAlternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release -
Research • MedicineSlow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions
Complex - MIC Roses and Liposomes Conservation, Solvent Evaporation Processes, Coacervation Polymerization in Suspension and Emulsion Methods for Preparing Drugs and Intermediates
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Research • Immunology and InfectionVisualization of Bacterial Resistance using Fluorescent Antibiotic Probes -
Research • BioengineeringPreparation, Purification, and Use of Fatty Acid-containing Liposomes -
Research • BioengineeringDelivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
Controlled release of drugs Systems for release of drugs in physiological control
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Research • BioengineeringMagnetic and Thermal-sensitive Poly(N-isopropylacrylamide)-based Microgels for Magnetically Triggered Controlled Release -
Research • BioengineeringSolid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications -
Research • BioengineeringFormulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
Preparation of tablets by the wet granulation method and the examination of the physicochemical properties of the tablets
