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通过通过纳米孔可调纳米易位流速Zeta电位测定：利用DNA修饰颗粒为例

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Please note that all translations are automatically generated. Click here for the English version.

通过通过纳米孔可调纳米易位流速Zeta电位测定：利用DNA修饰颗粒为例

DOI:

10.3791/54577-v

•

08:42 min

•

October 26, 2016

•

Emma L. C. J. Blundell, Robert Vogel³, Mark Platt

¹Department of Chemistry, School of Science,Loughborough University, ²Izon Science Limited, ³School of Mathematics and Physics,The University of Queensland

Chapters

00:05Title
01:07Preparing Streptavidin Coated Particles and Addition of Capture Probe DNA
02:45Hybridizing Complementary DNA to the Capture Probe-particles
03:46TRPS Setup
04:58Calibrating the Nanopore for Zeta Analysis and Running a Sample
06:43Results: Size and Zeta Potential of Streptavidin Coated Nanoparticles by TRPS
07:47Conclusion

Summary

Automatic Translation

在这里，我们使用集成到一个电阻脉冲感测技术经由粒子易位速度的测量，这可以被用来确定各个纳米粒子的ζ电位来表征纳米颗粒表面化学的聚氨酯可调谐纳米孔。

Tags

Keywords: Zeta Potential Nanoparticle Tunable Nanopore DNA-modified Particles TRPS Particle-by-particle Analysis Bioassay Streptavidin-coated Particles DNA Binding Magnetic Separation Target DNA Binding

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