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Chapters
- 00:05Title
- 00:42B and T Cell Purification
- 03:12Re-using the Separation Column
- 04:16CFSE Labeling and Stimulation
- 06:05Results: Representative Downstream Analyses of Magnetic Bead-purified T and B Cells
- 07:19Conclusion
Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.
