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JoVE Journal
Immunology and Infection

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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
 

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Article DOI: 10.3791/56689-v 10:35 min December 26th, 2017
December 26th, 2017

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Summary

Antibody-based drugs have revolutionized treatment for inflammatory diseases. In addition to having direct effects on specific targets, antibodies can activate macrophages to become anti-inflammatory. This protocol describes how anti-inflammatory macrophage activation can be assessed in vitro, using mouse bone marrow macrophages, and in vivo, using peritoneal macrophages.

Tags

Keywords: Antibody-based Drugs Murine Bone Marrow Peritoneal Macrophages Anti-inflammatory IL-10 Immunology Primary Macrophages Bone Marrow In Vitro Peritoneal Cavity In Vivo Euthanized Mouse Bone Marrow Isolation Bone Marrow Culture MCSF
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