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細菌でエチレン応答を研究するためのツールとして、エチレン放出化合物、2-クロロエチルホスホン酸を利用します
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
DOI:

08:51 min

November 10, 2016

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Chapters

  • 00:05Title
  • 00:45Triple Response Assay
  • 03:14Pellicle Assay
  • 07:03Triple Response Assay and Pellicle Assay Results
  • 08:02Conclusion

Summary

Automatic Translation

The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.

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