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Открыть Трахеостомия Желудочный кислота Аспирация мышиной модели острого повреждения легких Результаты в Максимальный Острый несмертельного повреждения легких
JoVE Journal
Medicine
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JoVE Journal Medicine
Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury
DOI:

09:16 min

February 26, 2017

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Chapters

  • 00:05Title
  • 01:06Tracheal Exposure
  • 02:54Intratracheal Instillation
  • 04:27Broncoalveolar Lavage and Lung Processing
  • 06:47Results: Representative Post-injury Histopathological and Albumin Leakage Analyses
  • 07:40Conclusion

Summary

Automatic Translation

This protocol induces acute lung injury in a mouse that has close fidelity to the pathogenesis of acid pneumonitis observed in humans. We generate a maximal acute nonlethal low pH lung injury and account for differences in rodent-human anatomic respiratory structure using an open tracheostomy coupled with circumferential pressure release.

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