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Chapters
- 00:05Title
- 00:35Cell Preparation
- 01:19Imaging
- 02:53Image Analysis
- 04:05Results: Behavior of Aggregation-prone Prion-like Proteins in D. discoideum during Heat Stress
- 05:25Conclusion
The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.
