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間接ニューロン、星状細胞共培養アッセイ:<em>インビトロ</em>ニューロングリアの相互作用の詳細な調査のためのセットアップ
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
DOI:

11:08 min

November 14, 2016

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Chapters

  • 00:05Title
  • 02:01Preparation and Cultivation of Primary Cortical Astrocytes
  • 05:19Preparing Astrocytes for the Indirect Coculture
  • 06:48Preparation of Primary Hippocampal Neurons
  • 09:03Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
  • 10:01Conclusion

Summary

Automatic Translation

このプロトコルは、ニューロン - グリア相互作用の区画化分析のための間接的なニューロン、星状細胞共培養を説明します。

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