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Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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1Department of Anesthesiology,Medical College of Wisconsin, 2Clement J. Zablocki Veterans Affairs Medical Center, 3Department of Surgery, Division of Pediatric Surgery,Children’s Research Institute, 4Department of Orthopedic Surgery,Medical College of Wisconsin, 5Deptarment of Anesthesiology,Clement J Zblocki Veteran Affairs Medical Center, 6Department of Medicine, Division of Cardiology,Medical College of Wisconsin
Chapters
- 00:05Title
- 01:05Facialis Artery Dissection
- 02:18Measuring Vasodilation in Isolated Artery
- 04:20Isolation of Cardiac Extracellular Matrix (ECM)
- 05:50Results: Treating Vessels and Cultured Cells with Decoy IRF5 Peptide, IRF5D
- 07:12Conclusion
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
