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Immunology and Infection

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JoVE Journal Immunology and Infection
A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
 

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

Article DOI: 10.3791/57361-v • 11:51 min • July 5th, 2018
July 5th, 2018 •
1, 1, 2, 3
1Department of Biochemistry, Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering, University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

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Summary
Automatic Translation

We have developed a multi-well format polyacrylamide-based assay for probing the effect of extracellular matrix stiffness on bacterial infection of adherent cells. This assay is compatible with flow cytometry, immunostaining, and traction force microscopy, allowing for quantitative measurements of the biomechanical interactions between cells, their extracellular matrix, and pathogenic bacteria.

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Keywords: Polyacrylamide Extracellular Matrix Stiffness Bacterial Infection Adherent Cells Host-pathogen Biomechanics Hydrogels Acrylamide Bis-acrylamide TEMED APS Fluorescent Microbeads
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