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Immunology and Infection
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JoVE Journal Immunology and Infection
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
 

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Article DOI: 10.3791/64425-v • 06:50 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee
* These authors contributed equally

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Summary
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A 96-well microtiter plate-based protocol using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-carboxanilide-2H-tetrazolium (XTT) reduction assay is described herein, to study antibodies' effects on biofilms formed by C. tropicalis. This in vitro protocol can be used to check the effect of potential new antifungal compounds on the metabolic activity of Candida species cells in biofilms.

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Biofilm Candida Tropicalis Antibody Antifungal Metabolic Activity Tetrazolium-based Reduction Assay 96-well Plate RPMI 1640 MOPS Medium PBS Wash Serum Dilution No-fungus Negative Control
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