基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响

DOI:

•

11:51 min

July 05, 2018

00:05Title
00:39Manufacturing Thin Two-layered Polyacrylamide Hydrogels on Multi-well Plates
01:36Polyacrylamide Hydrogel Fabrication
05:22Infection of Human Microvascular Endothelial Cells with L. monocytogenes
07:11Flow Cytometry to Quantify Extracellular-matrix-stiffness Dependent Susceptibility of Host Cells to Infection
08:02Quantitative Time-lapse Microscopy to Assess Extracellular-matrix-stiffness Dependent L. monocytogenes Dissemination Through Endothelial Cells
09:01Results: The Effect of Extracellular Matrix Stiffness on L. monocytogenes Infection of HMEC-1 Cells
10:32Conclusion

我们开发了一种多井格式的聚丙烯酰胺检测方法, 以探讨细胞外基质硬度对黏附细胞细菌感染的影响。该检测与流式细胞术、染色和牵引力显微镜相兼容, 可以定量测量细胞、胞外基质和致病细菌之间的生物力学相互作用。