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Microscopia de Luz Folha de fluorescência das raízes das plantas que crescem na superfície de um gel
JoVE Journal
Biology
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This content is Free Access.
JoVE Journal Biology
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
DOI:

06:41 min

January 18, 2017

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Chapters

  • 00:00Title
  • 01:24Plant Cultivation (Arabidopsis thaliana seedlings)
  • 02:04Plant Sample Preparation
  • 03:28Microscope Setup
  • 05:18Results
  • 06:00Conclusion

Summary

Automatic Translation

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

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