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Chapters
- 00:05Title
- 01:07Preparation and Photopolymerization of a PEGDA Hydrogel
- 03:23Preparing a Hydrogel for Microfluidic Network Formation via Laser-based Degradation
- 06:25Generating and Visualizing the Microfluidic Network
- 08:40Results: Comparing Fabricated Networks with Their In Vivo Counterparts and Initiating Fluid Flow
- 10:05Conclusion
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
