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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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Developmental Biology
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JoVE Journal Developmental Biology
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

DOI:
10.3791/55134-v

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09:16 min

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January 13, 2017

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,
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy,University of Newcastle

Chapters

  • 00:05Title
  • 00:51Mouse Embryonic Gonadal Ridge Isolation
  • 03:06Embryonic Gonadal Ridge Culture
  • 04:38Whole Mount Gonadal Ridge Immunofluorescence
  • 06:42Results: Wolffian Duct Morphogenesis and Surface Marker Expression
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

Tags

Organ CultureWhole Mount ImmunofluorescenceMouse Wolffian DuctsDevelopmental BiologyOrgan ShapeStructureFunctionIn VivoCell CultureNiche FactorsEmbryo DissectionUrogenital SystemWolffian DuctOrgan Culture Medium

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