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Journal / Medicine / Novel Diagnostics in Revision Arthroplasty:…
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JoVE Journal Medicine
Novel Diagnostics in Revision Arthroplasty
 

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Article DOI: 10.3791/55147-v • 10:35 min • December 3rd, 2017
December 3rd, 2017 •
1, 2, 2, 1, 2, 1, 3, 2
1Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, 3Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute

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Summary
Automatic Translation

Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.

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Keywords: Revision Arthroplasty Implant Sonication Multiplex Polymerase Chain Reaction Peri-prosthetic Joint Infection Joint Aspiration Microbiological Results Pathogen Detection Differential Diagnostics Joint Fluid Aspiration Blood Culture Flask PCR Analysis Cell Count EDTA
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